Rapid and quantitative detection ofLegionella pneumophilaapplying immunomagnetic separation and flow cytometry
نویسندگان
چکیده
منابع مشابه
Rapid detection of total and viable Legionella pneumophila in tap water by immunomagnetic separation, double fluorescent staining and flow cytometry
We developed a rapid detection method for Legionella pneumophila (Lp) by filtration, immunomagnetic separation, double fluorescent staining, and flow cytometry (IMS-FCM method). The method requires 120 min and can discriminate 'viable' and 'membrane-damaged' cells. The recovery is over 85% of spiked Lp SG 1 cells in 1 l of tap water and detection limits are around 50 and 15 cells per litre for ...
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ژورنال
عنوان ژورنال: Cytometry Part A
سال: 2010
ISSN: 1552-4922,1552-4930
DOI: 10.1002/cyto.a.20858